Journal: International Journal of Molecular Sciences

Article Title: Immunomodulatory Activity of Dietary Fiber: Arabinoxylan and Mixed-Linked Beta-Glucan Isolated from Barley Show Modest Activities in Vitro

doi: 10.3390/ijms12010570

Figure Lengend Snippet: IL-8 secretion from Caco-2 ( A ) and HT-29 ( B ) cells in response to treatment with fiber fractions extracted from the barley variety Tyra, zymosan and PMII (all 1 mg/mL). Cells were incubated with fiber of the respective fiber fractions in cell culture medium for 24 hours before IL-8 secretion was measured. Each bar represents the average ± SD of one representative experiment from a total of three independent experiments. * p < 0.05.

Article Snippet: Monoclonal mouse anti-human IL-8 antibody (BD Bioscience Pharmingen, San Diego, CA) suspended in coating buffer (0.1 M Carbonate/Bicarbonate buffer pH 9.6) was added to MaxiSorp TM ELISA plates (Nunc, Roskilde, Denmark) and incubated over night at 4 °C.

Techniques: Incubation, Cell Culture

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Schematic illustration of ( a ) the synthesis of bio-functionalized core-shell SERS nanotag; ( b ) the detection of IL-6 by core-shell SERS nanotags combined with the IFA kit.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques:

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Optimization of the SERS IFA conditions: ( a ) optimization of the amount of labelled antibody in the preparation of Au 4-MBA @Ag@Ab; ( b ) optimization of the amount of Au 4-MBA @Ag@Ab solution; ( c ) optimization of coating IL-6 antibody concentration on the NC membrane; ( d ) optimization of different washing times.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Concentration Assay

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: ( a ) Image of the test dot (4 mm in diameter) for different concentrations of IL-6; ( b ) averaged Raman spectra of test dot for different concentrations of IL-6 (A-H corresponding to 20 fg mL 〓1 -200 ng mL −1 ); ( c ) the calibration curve of IL-6, and the inset shown the linear part. The error bars are calculated from three measurements.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques:

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: Specificity of SERS IFA. ( a ) SERS spectra of Au 4-MBA @Ag at 1076 cm −1 , and ( b ) Raman intensity for IL-6, IL-8, IL-10, CRP, and SAA at a concentration of 100 pg mL −1 .

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Concentration Assay

Journal: Nanomaterials

Article Title: Quantitative Detection of Mastitis Factor IL-6 in Dairy Cow Using the SERS Improved Immunofiltration Assay

doi: 10.3390/nano12071091

Figure Lengend Snippet: ( a ) Western blot analysis of IL-6 protein spiked in the milk; ( b ) SERS spectra of different concentrations (0 ng mL –1 -1 µg mL −1 ) of IL-6 spiked in the milk and ( c ) the linear calibration curve; ( d ) comparison of detection results obtained from proposed method (red circles) and reference ECLIA method (black squares). Error bars are calculated from three measurements.

Article Snippet: Recombinant IL-6 and mouse anti-human IL-6 monoclonal antibody were commercially purchased from Beijing Key-bio Biotech Co., Ltd. (Beijing, China).

Techniques: Western Blot